Common Toxicity Testing


EnviroScience Laboratory Technician performs specialized toxicity testingOur Bioassay Laboratory provides comprehensive common toxicity tests to help clients comply with the Environmental Protection Agency (EPA) regulations, National Pollutant Discharge Elimination System (NPDES) permit requirements, and more.

EnviroScience provides high-quality environmental services to hundreds of satisfied municipal, industrial, and private-sector clients throughout the U.S.

Our excellent reputation with regulatory agencies on both the state and federal levels, our proven methodology, and the professionalism of our experienced biologists afford us confidence in guaranteeing our procedures and reported data will meet all acceptance criteria.

Primary Test Methods*

*Quotations for other test species and test types are available upon request.


Methods for Measuring Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms:


  • 48-hour Ceriodaphnia dubia (water flea); Method 2002.0
  • 48-hour Daphnia magna (water flea); Method 2021.0
  • 48-hour or 96-hour Pimephales promelas (fathead minnow); Method 2000.0
  • 48-hour Oncorhynchus mykiss (rainbow trout); Method 2019.0 Marine
  • 24-hour, 48-hour, & 96-hour Americamysis bahia (mysid shrimp); Method 2007.0
  • 48-hour Cyprinodon variegatus (sheepshead minnow); Method 2004.0


Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Water for Freshwater Organisms:

  • 7-day Ceriodaphnia dubia (water flea) survival and reproduction; Method 1002.0
  • 7-day Pimephales promelas (fathead minnow) growth and survival; Method 1000.096-hr.
  • 96-hour Selenastrum capricornutum (green algae) growth; Method 1003.0


Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Water for Marine and Estuarine Organisms:

  • 7-day Cyprinodon variegatus (Sheepshead minnow), survival and growth, Method 1004.0
  • 7-day Americamysis bahia (mysid shrimp); survival, growth, and fecundity; Method 1007.0

Other Tests Offered

Chronic Daphnia magna Life Cycle Toxicity Test

  • 21-day Daphnia magna (water flea) survival and reproduction; ASTM E 1193-97 (2004)

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    Few environmental firms in the country retain EnviroScience’s degree of scientific know-how, talent, and capability under one roof. The diverse backgrounds of our biologists, environmental engineers, scientists, and divers enable us to provide comprehensive in-house services and an integrated approach to solving environmental challenges—saving clients time, reducing costs, and ensuring high-quality results.

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